ProVen Professional – Study – The Lab4 and Immune Response Study
The Lab4 and Immune Response Study
This study examined the effect of a 12-week Lab4 probiotics and fructooligosaccharide supplementation programme on ex-vivo pro-inflammatory and anti-inflammatory cytokine production by peripheral blood mononuclear cells extracted from healthy volunteers.
- 20 healthy volunteers received a supplement comprising 25 billion Lab4 probiotics and 2g of fructooligosaccharide (FOS) daily for 12 weeks.
- Blood samples were collected at the start, 6 and 12 weeks.
- Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples and cultured ex-vivo in the absence ('resting') or presence (challenge) of lipopolysaccharide (bacterial toxin).
In the ‘resting’ immune state:
- Significant enhancement of production of the anti-inflammatory cytokines IL-10 and TBF-β over the 12 week period (**P<0.0001)
Fold change in IL-10 level
Fold change in TGF-β level
During challenge with bacterial toxin:
- Significant decrease in production of the pro-inflammatory cytokines IL-6 and IL-1β at both six weeks and 12 weeks (*P<0.01,**P<0.0001)
Fold change in IL-6 level
Fold change in IL-1β level
The consumption of Lab4 probiotics in combination with FOS alters ex vivo cytokine production demonstrating the potential immunomodulatory benefits.
Hepburn NJ, Garaiova I, Williams EA, Michael DR, Plummer S.
Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals.
Beneficial Microbes 2013, 4(4): 313-17
Human Nutrition Unit, Department of Oncology, University of Sheffield