Proven Professional – Study – The Cardiff Tracking Study
The Cardiff Tracking Study
This study evaluated if the colony fingerprint technique (RAPD) could be used to track probiotics Lactobacillus acidophilus CUL21 (from Lab4) and Lactobacillus salivarius CUL61 (from Lab4b) during consumption by healthy volunteers.
- The study was carried out at the School of Biosciences at Cardiff University.
- 12 healthy volunteers were given one capsule of Lactobacillus salivarius CUL61 (18 billion cfu per capsule) and Lactobacillus acidophilus CUL21 (5.6 billion cfu per capsule) once a day for 14 days.
- Faecal samples were collected before, during and after probiotic consumption.
- Lactic Acid Bacteria (LAB) isolates were genetically typed using a Random Amplified Polymorphic DNA (RAPD) typing scheme and the faecal samples were studied to identify if the LAB isolates L. salivarius CUL61 and L. acidophilus CUL21 were present before, during and after the probiotic capsule consumption.
- RAPD fingerprinting of the major colony types appearing after cultivation of each faecal sample was used to determine if the Lactobacillus strains had survived gastric and intestinal passage.
Detection of Lactobacillus salivarius CUL61 and Lactobacillus acidophilus CUL21 in faecal samples of healthy volunteers on day 7:
(A) L. salivarius CUL61 colonies from faecal sample grown on plate. Labelled as No. 6 and No.7
(B) L. acidophilus CUL21 colonies grown on plate. Labelled as No. 3, 4 and 5
(C) RAPD fingerprint technique:
Lanes 6, 7 - L. salivarius CUL61 from colonies are the same as L. salivarius - CUL61 control (Lane 1)
Lanes 3, 4, 5 - L. acidophilus CUL21 from colonies are the same as L. acidophilus - CUL21 control (Lane 2)