Other Study - Lactobacillus plantarum CUL66 and the Cholesterol Study

Lactobacillus plantarum CUL66 and the Cholesterol Study

Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes

Aim

To examine the cholesterol lowering ability of Lactobacillus plantarum CUL66 in an in vitro model of the intestinal epithelium.

Method

  • Cholesterol lowering ability and bile salts hydrolase (BSH) activity in L. plantarum CUL66 was assessed using in vitro models
  • The effect of L. plantarum CUL66 on cholesterol transport was assessed in an in vitro Caco-2 cell intestinal model

Results

1. Cholesterol removal

Cholesterol levels in bacterial culture media were lowered in the presence of L. plantarum CUL66 (**P=0.001)

2. Bile salt hydrolase activity

A white precipitate was observed when L. plantarum CUL66 was grown in the presence of bile salts, which is indicative of BSH activity

BSH activity

 3. Cholesterol transport

L. plantarum CUL66 significantly reduced the uptake of extracellular cholesterol by intestinal epithelial cells (**P<0.001)

L. plantarum CUL66 significantly reduced the expression of cholesterol transporter NPC1L1, which is critical for the uptake of cholesterol (*P=0.015)

L. plantarum CUL66 significantly reduced the efflux of intracellular cholesterol from intestinal epithelial cells into the basolateral (tissue) compartment (**P<0.001)

L. plantarum CUL66 significantly reduced the expression of cholesterol transporter ABCA-1, which is involved in the efflux of intracellular cholesterol to the basolateral (tissue) compartment (**P<0.001)

Summary

Lactobacillus Plantarum CUL66 Mechanism of Action

1. Cholesterol Removal

  • Cholesterol is bound or metabolised by CUL66 and subsequently excreted in faeces.

2. Bile salt hydrolase activity

  • Deconjugation (modifying) of bile acids leads to their reduced re-absorption and places an increased demand on the liver to synthesize more bile acids from circulating cholesterol in blood to replenish intestinal bile acids lost in faeces.

3. Reduction of cholesterol transport across the intestinal epithelium

  • 3a – reduction in the uptake of extracellular cholesterol and reduced expression of the NCP1L1 cholesterol transporter; 3b- reduction in the efflux of intracellular cholesterol to the basolateral compartment (tissues) and reduced expression of the ABCA-1 cholesterol transporter; 3c – no change on the intracellular cholesterol efflux back into the apical (intestinal lumen) compartment.

Conclusion

Lactobacillus plantarum CUL66 is a very promising cholesterol lowering candidate.

Reference

Michael DR et al.
Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes.
Beneficial Microbes 2016; 7(3):443-51

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